Coding

Part:BBa_J100238

Designed by: Nicholas Elder   Group: Campbell M Lab   (2015-11-09)

tCloneTetRed with short stuffer

This is the tConeTetRed insert (part BBa_J119386). The insert had the P2 promoter and GFP gene removed by digestion with BsaI and has had a short stuffer sequence (11bp) ligated in its place. This was used to test the effectiveness of tConeTetRed without the presence of a riboswitch, so the presence of the Tet-A RFP fusion protein is directly controlled by the P5 promoter. This was tested in JM109 E. coli cells and produces measurable fluorescence when grown in the presence of Tetracycline in a range of concentrations from 10 to 50 ug/mL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 462
    Illegal NgoMIV site found at 830
    Illegal NgoMIV site found at 990
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2049
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None